Storage of Heparinized Blood

Overestimation of plasma nonesterified fatty acid concentrations in heparinized blood.

Concentrations in Heparinized Blood To the Editor: With much interest have we read the important study on the use of glucose-insulin-potassium (GIK) in diabetic patients undergoing coronary artery bypass grafting by Lazar and coworkers.1 A potential mechanism underlying the beneficial effect of GIK administration includes metabolic modulation of (post)ischemic myocardium. Lowering of the plasma...

Physiologic Studies during Cardiopulmonary Bypass Eliminating Heparinized Blood.

A whole blood lymphocyte proliferation assay in healthy Thais: comparison of heparinized blood and acid citrate dextrose blood.

The lymphocyte proliferation assay (LPA) is a technique to determine T-lymphocyte functions in vitro. The standard LPA using peripheral blood mononuclear cells (PBMC) separated from heparinized blood requires a large blood sample, time consuming and expensive. It is more useful if acid citrate dextrose (ACD) blood could be used not only for LPA but also for other purposes. To determine whether ...

Evaluation of Blood Storage Lesions in Leuko-depleted Red Blood Cell Units

Background: Red blood cells (RBCs) undergo biochemical and morphologic alterations during storage that are known as the storage lesions causing decreased RBC quality and are correlated with transfusion reactions in certain groups especially in infants and critically ill patients. Microvesicles (MVs) as one of storage lesions may be derived from various cell types and have key roles in several...

Flow cytometric quantitation of phagocytosis in heparinized complete blood with latex particles and Candida albicans.

We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripheral blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1 micron diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leu...